Anti-MAG (myelin associated glycoprotein, GMA, Myelin-associated glycoprotein precursor, SIGLEC4A, S

Myelin-associated glycoprotein (MAG), also known as Siglec-4a, is a 70kD type I transmembrane glycoprotein in the sialoadhesin family. MAG is expressed on myelinating oligodenrocytes and Schwann cells and preferentially binds alpha 2, 3-linked sialic acid on O-linked glycans and gangliosides. MAG functions in the maintenance of oligodendrocytes and myelinated axons. , , Applications: , Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA (Capture): 2-8ug/ml. , Western Blot: 1-2ug/ml. The detection limit for rrMAG is ~25ng/lane under non-reucing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.