Anti-MAC, Human, mAb B7

W: A non-reduced or reduced sample treatment and SDS-Page was used to visualize C9. The band size is 71 kDa. IHC-P: Tissue sections were pretreated with a citrate antigen retrieval followed by pepsin (1 mg/ml) and blocked with 0.3% BSA + 15% normal goat serum. B7 staining was used at 10 µg/ml (overnight at 4oC). IA: The B7 antibody (1 µg/ml) was captured overnight at 4oC in PBS and blocked with 1% BSA + PBS-T. A polyclonal rabbit-anti-human C9 antibody was used as the detection (Ref 1,2).