Anti-LRRC4 (Leucine-rich Repeat-containing Protein 4, Brain Tumor-associated Protein BAG, Nasopharyn

LRRC4 also called NGL-2 (netrin-G ligand-2) or NAG14 (nasopharyngeal carcinoma- associated gene 14) is a 55kD type I transmembrane protein that is a member of the NGL family of synaptic LRR adhesion molecules. Human LRRC4 cDNA encodes 653aa that include a 38aa signal sequence, a 489aa extracellular domain (ECD), a 21aa transmembrane domain, and a 105aa cytoplasmic domain. The ECD contains nine LRRs aa74-288, a C2 type Ig like domain aa354-440, and a Thr-rich segment aa455-526. Within the ECD, human LRRC4 shares 98% aa identity with mouse and rat, 99% aa identity with canine and bovine, and 99.6% aa identity with equine LRRC4. It also shares 54-55% aa identity with family members LRRC4C/NGL-1 and LRRC4B/NGL-3, but each recognizes different ligands. LRRC4 is predominantly expressed in the brain on neurons and astrocytes as a ligand for netrin-G2 on the dendritic surface of synaptic neurons. It is proposed to regulate the formation of excitatory synapses via recruitment of PSD-95 to the cytoplasmic domain after aggregation of LRRC4 at the surface. It suppresses proliferation by downregulating cell signaling pathways, resulting in altered expression of cell cycle regulating proteins and delay at the late G1 phase. It is thus considered a tumor suppressor protein and is often downregulated in brain tumors, particularly gliomas. Forced expression of LRRC4 in tumor cells slows proliferation and promotes differentiation. Addition of soluable LRRC4 to cultured neurons reduces excitatory synapse formation. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.