Anti-LRP1B (low-density Lipoprotein Receptor-related Protein 1B, LRP-1B, LRP­DIT)

LRP-1B is a 600-620kD member of the LDLR family of proteins. It is expressed on vascular smooth muscle cells and likely neurons, and binds multiple molecules,including uPA,PAI-1,PDGFRb, uPAR, and Pseudomonasexotoxin.Itisconsideredatumor suppressor, and appears to block the generation of plasmin. Mature human LRP-1B is a 4579aa type I transmembrane (TM) glycoprotein. It contains a 4424aa extracellular domain (ECD) aa21-4444 plus a 132aa cytoplasmic region aa4468-4599. In the ECD, there are 32 LDLR class A repeats, 14 EGF-like repeats, and 36 LDLR class B repeats that show an interspersing pattern. In the cytoplasmic region, one NLS aa4468-4474 and two endocytosis signal motifs aa4492-4562 are found. LRP-B1 is cleaved in the Golgi by furin after Arg3957 to create a noncovalent heterodimer consisting of a 515kD ECD, and an 85-95kD TM segment. Either uncleaved LRP-1B, or the dissociated heterodimer can undergo additional processing, leading to cleavage of the 90kD TM segment after Lys4435. This creates a 21kD TM C-terminal fragment that can undergo further g-secretase processing to generate a cytoplasmic, 18kD peptide that translocates to the nucleus. Over aa21-244, human LRP-1B shares 81% aa identity with mouse LRP-1B. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.