Anti-Liver-expressed Antimicrobial Peptide 2 (LEAP-2)

Human genome contains numerous genes whose protein products are unknown in terms of structure, interaction partner, expression, and function. From human blood ultrafiltrate, a novel gene-encoded, cysteine-rich, and cationic peptide termed as liver- expressed antimicrobial peptide 2 (LEAP-2) together with several circulating forms of LEAP-2 differing in their amino-terminal length were identified, all containing a core structure with two disulfide bonds formed by cysteine residues in relative 1-3 and 2-4 positions. The largest native LEAP-2 form of 40aa residues is generated from the precursor at a putative cleavage site for a furin-like endoprotease. In contrast to smaller LEAP-2 variants, this peptide exhibited dose-dependent antimicrobial activity against selected microbial model organisms. , LEAP2: rat= 75aa, mouse= 76aa, human= 77aa, Human chromosome: 5q31.1. Protein homology between mose, rat and human LEAP2 is 99%. LEAP2 is expressed in liver, blood, urine and kidney. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: ELISA: 1:100,000 (50-100ng control peptide/well), Western Blot: 1-10ug/ml (chemiluminescence), Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.