Anti-Lifeguard (Faim2, Fas Apoptotic Inhibitory Molecule 2, Protein Lifeguard, Transmembrane BAX Inh

LFG, a seven transmembrane spanning protein, is a recently identified member of a family whose members can inhibit apoptotic cell death induced by Fas receptor. LFG is a human homolog of rat NMP35 and consists of a conserved transmembrane domain and a small cytoplasmic domain. Reports have shown that LFG protects the cell from Fas-mediated neuronal cell death in a novel way. LFG binds to Fas receptor but doesn't have any effect in reducing Fas expression or decreasing the ability of FADD binding to the receptor. Furthermore, LFG doesn't protect the cells from TNF-alpha mediated cell death process. Although LFG is ubiquitously expressed, Northern blot analysis detected an increased expression in hippocampus and other neural tissues, suggesting a protective role in neurodegenerative diseases. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1-3ug/ml, Immunohistochemistry (Paraffin): 10ug/ml, Optimal dilutions to be determined by the researcher. Positive Control: Human, mouse and rat brain lysate, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.