Anti-LGR5 / Leucine-rich repeat-containing G-protein coupled receptor 5

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. LGR5 antibody detects Leucine-rich repeat-containing G-protein coupled receptor 5, a stem-cell marker and receptor for R-spondin proteins that potentiate Wnt/beta-catenin signaling. LGR5 is essential for maintaining self-renewing adult stem cell populations in the intestine, hair follicle, and stomach. The LGR5 antibody is used in stem-cell biology, oncology, and regenerative medicine to identify proliferative stem cell compartments.LGR5 is encoded by the LGR5 gene on human chromosome 12q21.1. The receptor is approximately 907 amino acids long, with seven transmembrane domains typical of GPCRs and a large extracellular region containing leucine-rich repeats that mediate ligand binding. Upon R-spondin binding, LGR5 interacts with Frizzled and LRP5/6 receptors, amplifying Wnt signaling to sustain stem-cell proliferation.The LGR5 antibody detects a 100-120 kilodalton protein by western blot and exhibits membrane and cytoplasmic localization in proliferative epithelial zones. In the intestine, LGR5 marks crypt base columnar cells capable of regenerating the entire epithelium. In the hair follicle, it labels bulge stem cells responsible for hair cycle renewal.LGR5 expression is tightly regulated by Wnt activity and is a key marker for cancer stem cells in colorectal and gastric carcinomas. Overexpression promotes tumor initiation and therapy resistance, while knockdown suppresses proliferation and restores differentiation. The receptor modulates beta-catenin stabilization, transcription of target genes, and maintenance of undifferentiated states.Because of its role in tissue regeneration and cancer stemness, LGR5 is an important biomarker for stem-cell tracking and targeted therapy development. NSJ Bioreagents provides a validated LGR5 antibody optimized for its applications, enabling precise identification of Wnt-responsive stem cell populations and cancer progenitors.