Anti-LGALS7/7B / Galectin 7

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. LGALS7/7B antibody detects Galectin-7 (encoded by LGALS7 and its paralog LGALS7B), a beta-galactoside-binding lectin that regulates epithelial differentiation, apoptosis, and tissue repair. The Galectin-7 protein belongs to the galectin family, which is defined by its conserved carbohydrate recognition domain (CRD) that binds beta-galactosides on glycoproteins and glycolipids. Encoded primarily by LGALS7 on chromosome 19q13.2, and to a lesser extent by LGALS7B (a highly similar duplicate), Galectin-7 acts as both an intracellular modulator of cell survival and an extracellular signaling molecule influencing cell adhesion and immune response.Galectin-7 is expressed abundantly in stratified epithelia, including the skin, cornea, and mucosal linings. It plays an important role in maintaining epithelial homeostasis by regulating keratinocyte differentiation and apoptosis. Upon tissue injury or stress, Galectin-7 is upregulated and released into the extracellular matrix, where it modulates cell-cell adhesion and migration during wound healing. It binds to extracellular glycoproteins such as integrins and laminins, mediating epithelial remodeling and cell communication. In keratinocytes, Galectin-7 localization shifts between the cytoplasm and nucleus depending on differentiation and stress conditions.At the cellular level, Galectin-7 interacts with intracellular proteins such as Bcl-2, promoting mitochondrial apoptosis under proapoptotic stimuli. It also influences p53-dependent transcriptional regulation of cell death genes. Through these pathways, Galectin-7 acts as a proapoptotic factor in damaged epithelial cells while promoting tissue regeneration. Overexpression of Galectin-7 enhances apoptosis and accelerates wound closure, whereas its loss impairs epithelial renewal and barrier integrity.The LGALS7/7B antibody is widely used in epithelial biology, dermatology, and oncology research to study differentiation, apoptosis, and cell adhesion. Western blot analysis identifies a 15 kilodalton band corresponding to Galectin-7, and immunohistochemistry reveals strong cytoplasmic and nuclear staining in epithelial layers. This antibody enables analysis of Galectin-7 distribution in normal and neoplastic tissues, supporting studies of epithelial morphogenesis and repair.In cancer, Galectin-7 shows context-dependent functions-it can suppress tumor progression in certain epithelial cancers by promoting apoptosis but can also enhance metastasis in others by regulating cell migration and extracellular matrix interactions. Altered expression has been reported in breast, cervical, and gastric cancers. The LGALS7/7B antibody is a critical reagent for understanding how glycan-binding proteins contribute to cancer cell plasticity, metastasis, and immune regulation. NSJ Bioreagents provides this antibody validated for its applications, ensuring reliable detection in epithelial and cancer research applications.