Anti-Latexin (Endogenous Carboxypeptidase Inhibitor, ECI, Protein MUM, Tissue Carboxypeptidase Inhib

Latexin, the only endogenous specific inhibitor known for zinc-dependent metallocarboxypeptidases, is a 222aa protein belonging to the protease inhibitor I47 (latexin) family. Human latexin is an elongated molecule with N- and C-terminal domains that each consist of an alpha helix enveloped by a curved beta sheet and the two domains are separated by a connecting segment. Latexin is a hardly reversible, non-competitive, and potent inhibitor of CPA1, CPA2 and CPA4 but is less potent against other mammalian carboxypeptidases, and it does not inhibit enzymes of other carboxypeptidase classes like metallopeptidases or serine proteases. In rat, Latexin may be used as a molecular marker for the regional specification of the neocortex. It is expressed in most tissues with high expression in brain, lung and digestive tract. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.01-0.1ug/ml, Immunohistochemistry (formalin fixed paraffin embedded): 10ug/ml, Optimal dilutions to be determined by the researcher. Positive Control: Human pancreas lysate, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.