Anti-Lat / Linker for activation of T cells

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. LAT antibody detects Linker for activation of T cells, a critical transmembrane adaptor protein that functions as a central scaffold in T-cell receptor (TCR) signaling. The UniProt recommended name is Linker for activation of T cells (LAT). This protein coordinates the assembly of multiprotein complexes that mediate T-cell activation, proliferation, and immune response regulation. LAT is indispensable for coupling TCR engagement to downstream signaling cascades that drive cytokine production and effector cell differentiation.Functionally, LAT antibody identifies a 233-amino-acid membrane protein that localizes to lipid rafts of the plasma membrane. Upon TCR stimulation, the Src-family kinase Lck and the ZAP-70 tyrosine kinase phosphorylate multiple tyrosine residues on LAT, creating docking sites for SH2-domain-containing proteins such as Grb2, PLC-gamma1, and Gads. These interactions recruit downstream effectors that activate calcium flux, MAPK signaling, and transcriptional pathways including NFAT, NF-kappaB, and AP-1. Through these mechanisms, LAT orchestrates the signal amplification necessary for full T-cell activation.The LAT gene is located on chromosome 16p11.2 and encodes a single-pass type III transmembrane protein with a short extracellular domain and a cytoplasmic tail rich in tyrosine residues. The LAT scaffold acts as an organizational hub, integrating signals from the TCR, co-stimulatory receptors, and cytokine receptors. LAT phosphorylation dynamics are tightly regulated to prevent aberrant T-cell activation and autoimmunity. In developing thymocytes, LAT signaling contributes to both positive and negative selection, shaping the mature T-cell repertoire.Defects in LAT expression or phosphorylation impair T-cell development and lead to severe combined immunodeficiency-like phenotypes. Conversely, gain-of-function mutations or dysregulated LAT signaling can cause hyperactivation syndromes, lymphoproliferative disorders, or autoimmunity. LAT also functions in mast cells, natural killer cells, and platelets, where it mediates Fc receptor-dependent signaling and degranulation responses.LAT antibody is widely used in immunology, signal transduction, and cell biology research. It is suitable for western blotting, flow cytometry, and immunofluorescence to examine TCR-induced phosphorylation and signal complex formation. Researchers employ this antibody to dissect T-cell activation pathways and study immune regulation at the molecular level. LAT serves as a key marker of functional TCR signaling in both primary immune cells and model systems.Structurally, LAT contains a cytoplasmic region with multiple phosphorylation sites that function as SH2-binding motifs for signaling partners. It forms large signalosomes through multivalent interactions that promote phase-separated clusters at the membrane. NSJ Bioreagents provides LAT antibody reagents validated for use in T-cell activation, immune signaling, and phosphorylation research.