Anti-LAT (36kD Phospho-tyrosine Adapter Protein, LAT1, Linker for Activation of T-cells Family Membe

LAT (Linker for activation of T cells, also pp36) is a 36-38kD type III (no signal sequence, extracellular N-terminus) transmembrane adaptor phosphoprotein. It is expressed on mast cells, NK cells, monocytes (but not macrophages), platelets and T cells. LAT is phosphorylated upon ligation of the TcR, GPVI, and FceRI, and serves as an anchor for several SH2-domain containing signaling proteins, including PLCg1, SLP76 and Grb2. Human LAT is 262aa in length. It contains a four aa extracellular segment with a 235aa cytoplasmic domain (aa28-262). Palmitoylation occurs on Cys26 and 29. There are 13 Ser/Thr phosphorylation sites plus five Tyr sites. Multiple potential splice variants are reported. There is an alternative start site 36aa upstream of the standard site that may be accompanied by a deletion of aa114-142. There is also a deletion of aa88-97, and three substitutions: two aa for aa215-262, 38aa for aa55-262, and 50aa for aa35-262. Over aa30-113, human LAT shares 73% aa identity with mouse LAT. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.