Anti-KYNU (Kynureninase, L-kynurenine Hydrolase)

Kyureninase (KYNU, a hydrolase that acts on dog [Greek: kyon] urine [Greek: ouron]) is a 50­55kD member of the kyureninase family of enzymes. It is a pyridoxal phosphate­dependent cytosolic hydrolase that occurs in a variety of cell types, including hepatocytes and macrophages. Kyureninase participates in the metabolism of tryptophan. Dietary Trp is either incorporated into protein, or metabolized into niacin, serotonin or NAD. Kyureninase catalyzes one of two steps that lead to the formation of a key downstream intermediate called 3­hydroxyanthranilic acid. Mouse Kyureninase is 464 amino acids (aa) in length. It contains one large catalytic site (aa24­462), and typically functions as a noncovalent homodimer. There are at least two potential isoform variants. One contains a three aa substitution for aa425­464, while another possesses a 22aa substitution for aa302­464. Full­length mouse Kyureninase shares 92% and 83% aa sequence identity with rat and human Kyureninase, respectively. Applications: Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml, Immunohistochemistry: 8­25ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.