Anti-Keratin-19, clone 0.N.365

Cytokeratin 19 is a member of type I acidic subfamily of intermediate filaments. it is expressed in various different human tissues except in liver. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis. Applications: Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Formalin/paraffin): 1:100-1:200 for 20 minutes at RT. Staining of formalin/paraffin tissues is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. (Protease XXIV damages the epitope recognized by this antibody)., Western Blot: 2-4ug/ml for 2 hours at RT., Optimal dilutions to be determined by the researcher. Positive Control: LS174T and HT29 cells, skin, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.