Anti-IRX1 / Iroquois-class homeodomain protein

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. IRX1 antibody detects Iroquois-class homeodomain protein IRX1, encoded by the IRX1 gene on chromosome 5p15.33. IRX1 is a transcription factor belonging to the Iroquois homeobox gene family, which regulates embryonic patterning, neuronal differentiation, and organogenesis. IRX1 contains a conserved homeodomain that binds DNA and directs gene expression programs critical for tissue development. In vertebrates, IRX1 contributes to forebrain, heart, limb, and craniofacial patterning. By modulating downstream effectors, it integrates positional cues during embryogenesis, ensuring correct morphogenesis.IRX1 expression persists in select adult tissues, suggesting roles beyond development, including neuronal plasticity and epithelial regulation. Dysregulation of IRX1 has been linked to oncogenesis. Tumor suppressor roles are observed in gastric cancer, where IRX1 downregulation correlates with poor prognosis, while in other cancers it may function as an oncogene depending on cellular context. Epigenetic silencing of IRX1 through promoter methylation has been reported, highlighting transcriptional repression as a mechanism in cancer progression. IRX1 antibody therefore provides researchers with a means to examine both developmental biology and disease-associated misregulation.At the molecular level, IRX1 acts as a transcriptional regulator of Wnt, BMP, and Notch signaling pathways, impacting proliferation, differentiation, and apoptosis. In cardiac development, IRX1 contributes to ventricular chamber specification and conduction system maturation. In neural development, it regulates differentiation of specific neuronal subtypes. Animal models with IRX1 knockouts display craniofacial abnormalities, cardiac defects, and disrupted hindbrain segmentation, highlighting its multifaceted role.Experimentally, IRX1 antibody has applications in immunohistochemistry, immunofluorescence, and western blotting. It is used to track IRX1 expression during embryogenesis, to validate promoter methylation effects in tumors, and to study transcription factor localization in nuclei. NSJ Bioreagents provides IRX1 antibody to facilitate investigations into morphogenetic regulation, cancer epigenetics, and gene regulatory networks.