Anti-IQSEC2 / IQ motif and Sec7 domain-containing protein 2

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. The IQSEC2 antibody targets IQ motif and Sec7 domain-containing protein 2, a guanine nucleotide exchange factor (GEF) encoded by the IQSEC2 gene. This multidomain protein belongs to the IQSEC/BRAG family of Arf GEFs, which regulate small GTPases of the ADP-ribosylation factor (Arf) family. IQ motif and Sec7 domain-containing protein 2 is primarily expressed in the brain, where it contributes to synaptic signaling, dendritic spine morphogenesis, and neuronal communication. The IQSEC2 antibody provides a reliable tool to examine the localization, regulation, and signaling roles of this neuron-specific exchange factor.IQ motif and Sec7 domain-containing protein 2 contains an IQ motif that binds calmodulin and a Sec7 catalytic domain responsible for activating Arf6. Through these structural features, IQSEC2 regulates membrane trafficking and actin cytoskeleton dynamics at excitatory synapses. Its activity influences AMPA receptor endocytosis, synaptic plasticity, and overall neuronal connectivity. The IQSEC2 antibody enables researchers to visualize these molecular processes, helping clarify how alterations in Arf6 activation impact synaptic function and brain development.Mutations in IQSEC2 are associated with X-linked intellectual disability, autism spectrum disorder, and epileptic encephalopathy. These pathogenic variants disrupt GEF activity or protein stability, resulting in abnormal neuronal signaling and synapse formation. By using the IQSEC2 antibody, investigators can detect wild-type and mutant protein forms in patient-derived cells, animal models, or neuronal cultures to better understand the molecular pathology underlying neurodevelopmental disorders. Analysis of IQ motif and Sec7 domain-containing protein 2 expression using this antibody provides insight into how altered GTPase signaling contributes to cognitive impairment and epilepsy.The IQSEC2 antibody is suitable for western blotting, immunocytochemistry, and immunohistochemistry applications. It reveals strong neuronal localization, particularly in the postsynaptic density and dendritic compartments. The reagent supports studies on synaptic vesicle recycling, endocytic trafficking, and Arf-dependent signaling. Combined with electrophysiology or live-cell imaging, the IQSEC2 antibody allows correlation of protein expression with functional outcomes in neuronal communication and plasticity.Beyond neuroscience, IQ motif and Sec7 domain-containing protein 2 may influence cytoskeletal remodeling in other cell types, including epithelial and immune cells, suggesting broader physiological relevance. NSJ Bioreagents provides the IQSEC2 antibody to facilitate ongoing exploration of how this key regulatory enzyme integrates calcium signaling, actin dynamics, and membrane trafficking. Its validated specificity ensures accurate quantification and localization across multiple experimental systems. The availability of this antibody supports research into synaptic biology, cognitive function, and mechanisms of neurodevelopmental disease.