Anti-IQCE / IQ domain-containing protein E

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. IQCE antibody recognizes IQ domain-containing protein E, a calcium-regulated component essential for the assembly and stability of primary cilia. IQCE belongs to a family of IQ motif-containing proteins that bind calmodulin and function in calcium signaling regulation. Within ciliated cells, IQCE localizes at the basal body and transition zone, helping to anchor structural and signaling complexes necessary for ciliogenesis. The IQCE antibody is a critical reagent for exploring developmental biology, signaling regulation, and ciliopathies caused by defective ciliary assembly.IQCE is encoded by the IQCE gene located on human chromosome 7p22.3. The protein contains one or more IQ motifs, short consensus sequences that mediate calcium-calmodulin interactions. These motifs enable IQCE to sense calcium changes and respond by altering its binding partners and structural conformation. It interacts with EF-hand calcium-binding protein EFCAB1 to form a complex required for the stability of the ciliary base. This partnership regulates Hedgehog signaling, a pathway central to embryogenesis and tissue differentiation. Disruption of IQCE causes ciliopathies, including acrocallosal syndrome, characterized by brain malformation, craniofacial defects, and polydactyly.With the IQCE antibody, western blot analysis typically reveals a 77 kilodalton band, while immunofluorescence shows punctate staining at centrosomes and basal bodies. Ciliary localization is confirmed through co-staining with gamma-tubulin and acetylated tubulin. Loss-of-function studies demonstrate that IQCE depletion shortens or eliminates primary cilia, disrupting Hedgehog target gene activation. The protein also supports the recruitment of intraflagellar transport (IFT) components, ensuring proper axonemal elongation.Beyond ciliary biology, IQCE expression in epithelial and fibroblast cells suggests additional roles in vesicular trafficking, cytoskeletal organization, and mechanosensory signaling. Its calcium sensitivity allows IQCE to integrate mechanical and biochemical cues, influencing organ development and tissue maintenance. NSJ Bioreagents provides a validated IQCE antibody optimized for western blot, immunofluorescence, and confocal imaging applications. This reagent enables detailed exploration of calcium-calmodulin signaling and the molecular basis of ciliary assembly in health and disease.