Anti-Interleukin 33 (IL-33)

IL-33 is a member of the IL-1 cytokine family and is constitutively expressed in smooth muscle cells and the airway epithelium. IL-33 signals through receptors, IL-1RL1 and IL-1RAP, to induce type 2 cytokine production in polarized Th2 cells. Applications: Suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry, ELISA, Immunohistochemistry and Neutralization. Other applications not tested. Recommended Dilutions: Western Blot: 0.4ug/ml using HEK293T human embryonic kidney cell line transfected with full length mouse IL-33., Flow Cytometry (Intracellular): 2.5ug/10e6 mouse splenocytes treated with PMA and Ca2+ ionomycin, fixed with paraformaldehyde and permeabilized with saponin., Immunocytochemistry: 5-15ug/ml using Immersion fixed bEnd. mouse endothelioma cell line, Immunohistochemistry: 5-15ug/ml using perfusion fixed frozen sections of mouse spleen, ELISA (Capture): 0.2-0.8ug/ml, Neutralization: 10-50ng/ml in the presence of 0.25ng/ml of recombinant mouse IL-33., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.