Anti-Interleukin 17F (IL-17F)

The Interleukin 17 family proteins, comprising six members (IL-17A through IL-17F), are secreted, structurally related proteins that share a conserved cystine knot fold near the C­terminus, but have considerable sequence divergence at the N­terminus. With the exception of IL­17B, all IL­17 family members are disulfide­linked dimers. IL­17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions. Human IL-17F cDNA encodes a 163aa protein with a putative 30aa signal peptide. Among IL­17 family members, IL­17F is most closely related to IL­17A (~44% aa sequence homology), but shares only limited sequence homology (16-30%) with IL­17B, C, D and E. Human and mouse IL­17F share 55% sequence identity. IL­17F is expressed in activated CD4+ T­cells and activated monocytes. Although the ligands for IL­17 RD and E are not known yet, it is reported that IL­17 RA binds IL­17A, and IL­17 RB binds IL­17B and IL­17E. IL­17 RC binds IL­17A and IL­17F with similarly high affinity and functions as a receptor for both IL­17A and IL­17F (5, 6). IL­17F stimulates production of IL­6, IL­8, G­CSF and regulates cartilage matrix turnover by increasing matrix release and inhibiting new matrix synthesis. IL­17F also inhibits angiogenesis and induces production of IL­2, TGF­b, and monocyte chemoattractant protein­1 in endothelial cells. Applications: Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA (Capture): 0.2-0.8ug/ml, Western Blot: 0.1-0.2ug/ml The detection limit is ~5ng/lane under non-reducing and reducing conditions., Immunocytochemistry: 15ug/ml , Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.