Anti-Integrin, beta7 (Integrin BetaP, M290 IEL Ag)

Human Integrin Beta7 (also Integrin BetaP and M290 IEL Ag) is a 120kD member of the Integrin beta chain family of molecules. It is a type I transmembrane glycoprotein that contains a 704 amino acid (aa) extracellular domain (ECD) (aa20-723) and a 52aa cytoplasmic tail. The ECD contains one von Willebrand factor A domain (aa150-389) and four cysteine-rich EGF-like repeats. One splice variant exists that shows a deletion of aa501-648. Integrin Beta7 forms a nondisulfide linked heterodimer with alpha4 and alphaE integrins. Alpha4Beta7 binds fibronectin, VCAM-1 and MADCAM1, while alphaEBeta7 binds E-cadherin. The ECD of human Beta7 is 87% aa identical to the ECD of mouse Beta7. Applications: Suitable for use in Direct ELISA, Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilutions: Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~0.2ng/well., Western Blot: 0.1-0.2ug/ml. The detection limit is ~5ng/lane under non-reducing and reducing conditions., Flow Cytometry: 25ug/ml. 10ul labels 1-2.5x10e5 cells in a total reaction volume <200ul. Tested using blood derived lymphocytes., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.