Anti-Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)

Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system. It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilatation by releasing NO from the endothelium. Unlike typical neurotransmitter, NO is a not stored in synaptic vesicle and does not act on membrane receptors. Synthesis of NO, initially demonstrated in vascular endothelium, is now found in many tissues. Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunofluorescence: 1:10, Western Blot: 1:40 detects a singe band at ~130kD., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.