Anti-IL33 (IL1F11, Interleukin-33, IL-33, Interleukin-1 Family Member 11, IL-1F11, Nuclear Factor fr

IL-33 is a 270aa highly divergent protein belonging to the IL-1 family with an IL-1-like C-terminal domain. It is a dual function protein that may function both as a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. It binds to and signals through IL1RL1:ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6. It activates NF-kappaB and MAP kinases, and drives production of TH2-associated cytokines from in vitro polarized TH2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs. It is proteolytically converted to a mature form by CASP1 and is highly expressed in high endothelial venules found in tonsils, Peyer's patches and mesenteric lymph nodes and is almost undetectable in placenta. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml, Optimal dilutions to be determined by the researcher. Positive Control: HUVEC cell lysate, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.