Anti-IL10 / Interleukin 10, clone AOIG-9

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. IL10 antibody detects Interleukin 10, a pleiotropic anti-inflammatory cytokine that suppresses immune activation and maintains immune homeostasis. Encoded by the IL10 gene on chromosome 1q32.1, this cytokine is secreted primarily by monocytes, T regulatory cells, macrophages, and B cells. IL10 acts by limiting the expression of proinflammatory cytokines, antigen presentation, and costimulatory molecules, thereby protecting tissues from excessive immune-mediated damage.Interleukin 10 functions through binding to the IL10 receptor complex, composed of IL10RA and IL10RB subunits, triggering activation of JAK1 and TYK2 kinases. This leads to phosphorylation of STAT3, which translocates to the nucleus to induce anti-inflammatory gene expression. Targets include SOCS3, IL1RA, and other regulatory molecules that dampen NF-?B-mediated transcription of inflammatory mediators such as TNF, IL1, and IL6. IL10 signaling is essential for balancing immune tolerance with pathogen defense.The IL10 antibody is widely used in immunology, inflammation, and infectious disease research to study cytokine signaling, immune regulation, and autoimmune disorders. Western blot analysis detects a 19 kilodalton band corresponding to IL10, while ELISA and immunofluorescence applications reveal secretion patterns and localization in immune cell cultures. This antibody supports detailed characterization of IL10 expression in response to infection, inflammation, and therapeutic modulation.Deficiency or dysregulation of IL10 signaling leads to chronic inflammation and autoimmune pathologies, including inflammatory bowel disease, rheumatoid arthritis, and psoriasis. Conversely, elevated IL10 levels in tumors and chronic infections can suppress immune responses and promote immune evasion. Therapeutic modulation of IL10 signaling is under investigation for treating autoimmune and inflammatory diseases, highlighting its dual protective and suppressive roles. The IL10 antibody provides a reliable tool for tracking cytokine expression, validating immunomodulatory pathways, and developing anti-inflammatory therapies. NSJ Bioreagents offers this antibody validated for its applications, ensuring high-quality performance in immune signaling research.