Anti-IgA (mouse) Rabbit Monoclonal Antibody - Biotinylated (RM220)

Immunoglobulin A (IgA) is a member of the immunoglobulin superfamily of glycoproteins with roles in host defense against intestinal pathogens and both quantitative and qualitative control of host commensal microbiota composition. It is produced by B cells and later secreted by plasma cells and is the most abundant antibody on mucosal surfaces that comprises at least 70% of all Ig produced in mice. Mouse IgA consists of two heavy chains of approximately 53.5 kDa each and two light chains of approximately 25 kDa each. Unlike human IgA, mouse IgA exists as a single isotype and is primarily found as a dimer that lacks the disulfide bonds between the light and heavy chains present in other Ig classes. Production of IgA is induced in the gut only in animals containing intestinal microbes, and the number of IgA-producing plasma cells is reduced in germ-free mice. IgA-deficient mice exhibit increased lethality compared with wild-type mice in a model of influenza infection, as well as reduced parasite clearance in a model of G. muris infection. However, IgA deficiency does not affect clearance of vaginal infection with herpes simplex virus 2 (HSV-2), indicating redundancy in pathogen protection with compensation by antibodies of other isotypes or innate immune mechanisms at mucosal surfaces. Cayman's IgA (mouse) Rabbit Monoclonal Antibody - Biotinylated (RM220) can be used for ELISA and Western blot (WB, non-reducing conditions) applications. The antibody recognizes IgA from mouse samples.Synonyms: Immunoglobulin A. Immunogen: Mouse IgA. Clone Designation: RM220. Formulation: (Request formulation change), 50 µg of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ELISA, WB (non-reducing conditions). Cross Reactivity: (+) IgA, (-) Mouse IgG1, (-) IgG2a, (-) IgG2b, (-) IgG2c, (-) IgG3, (-) IgM, (-) IgE, (-) Human IgA.