Anti-HMGB1, CT (HMGB1, HMG1, High mobility group protein B1, High mobility group protein 1)

DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells (By similarity). Applications: Suitable for use in Western Blot, Immunohistochemistry, ELISA. Recommended Dilution: ELISA: 1:1,000, Western Blot: 1:500~1600, Immunohistochemistry: 1:10~50, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.