Anti-Histone H3K9Me3 Monoclonal Antibody (RM389)

Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei. It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination. Trimethylation of histone H3 at lysine 9 (H3K9Me3) occludes DNA binding by transcription factors and is associated with gene repression. H3K9Me3 is also a binding target of the heterochromatin protein 1 (HP1) family proteins CBX1, CBX3, and CBX5. It is recognized by the plant homeodomain (PHD) fingers of chromodomain helicase DNA binding protein 4 (CHD4), a member of the NuRD deacetylase complex that is upregulated in a variety of cancers and implicated in the DNA damage response. Cayman's Histone H3K9Me3 Monoclonal Antibody (RM389) can be used for ELISA, multiplex-based assays, and Western blot (WB) applications.Synonyms: Trimethylated Histone H3 Lysine 9. Immunogen: Peptide corresponding to H3K9Me3. Clone Designation: RM389. Formulation: (Request formulation change), 100 µg of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ELISA, multiplex-based assays, WB. Cross Reactivity: (+) H3K9Me3, (-) Unmodified H3K9, (-) H3K9Me1, (-) H3K9Me2, (-) H3K4Me1, (-) H3K4Me2, (-) H3K4Me3, (-) H3K27Me1, (-) H3K27Me2, (-) H3K27Me3, (-) H3K36Me1, (-) H3K36Me2, (-) H3K36Me3, (-) H4K20Me1, (-) H4K20Me2, (-) H4K20Me3.