Anti-Histone H3.3, clone RM190 (recombinant antibody)

1 mg/ml in PBS with 50% Glycerol, 1% BSA and 0.09% sodium azide. This recombinant Histone H3.3 antibody reacts to Histone H3.3, independent of post-translational modifications. No cross reactivity with Histone H3.1 or other histone proteins. Protein function: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. [The UniProt Consortium]