Anti-Histone H3 (tri methyl K27) antibody / HIST1H3A, clone 32H02

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. Histone H3 (tri methyl K27) antibody detects histone H3 trimethylated at lysine 27 (H3K27me3), a hallmark of transcriptional repression and epigenetic silencing. Histone H3, encoded in part by HIST1H3A, is a core component of nucleosomes that package DNA into chromatin. Trimethylation of K27 is catalyzed primarily by the polycomb repressive complex 2 (PRC2), where EZH2 acts as the methyltransferase. This modification recruits polycomb group proteins to establish long-term gene silencing, influencing cell identity and development.Research using Histone H3 (tri methyl K27) antibody highlights its importance in developmental biology. H3K27me3 marks promoters of genes that regulate differentiation, allowing pluripotent stem cells to maintain plasticity while silencing lineage-specific programs. During differentiation, changes in H3K27me3 distribution establish lineage-specific transcriptional landscapes. This modification is also involved in X-chromosome inactivation, imprinting, and developmental timing.Aberrant H3K27 methylation is strongly implicated in cancer. Overexpression or mutation of EZH2 leads to abnormal accumulation of H3K27me3, silencing tumor suppressor genes and promoting malignancy. Conversely, loss of H3K27me3 due to mutations in PRC2 subunits can activate oncogenes and alter differentiation. Elevated or reduced H3K27me3 levels have been reported across lymphomas, sarcomas, and solid tumors, making it both a biomarker and therapeutic target. EZH2 inhibitors are now in clinical use, with antibody-based detection of H3K27me3 serving as a critical biomarker of drug activity.In neurobiology, abnormal H3K27me3 patterns have been associated with neurodevelopmental syndromes, intellectual disability, and psychiatric conditions. Inflammatory pathways are also modulated by this histone mark, where silencing of cytokine promoters helps control immune responses.Antibodies against H3K27me3 are validated for chromatin immunoprecipitation (ChIP), immunofluorescence, immunohistochemistry, and western blot. These reagents allow mapping of epigenetic silencing across genomes, supporting research in development, disease, and therapeutic interventions. Clone-based antibodies ensure high specificity for the trimethylated lysine, avoiding cross-reactivity with other methylated or acetylated residues.NSJ Bioreagents provides this Histone H3 (tri methyl K27) antibody for epigenetics and cancer biology studies.