Anti-Histone H3 (di methyl K4) / HIST1H3A, clone 31H99

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. Histone H3 (di methyl K4) antibody detects histone H3 when lysine 4 is di methylated, a key epigenetic modification that influences chromatin structure and gene expression. Histones are core proteins that package DNA into nucleosomes, and post translational modifications of histones regulate access of transcriptional machinery to genomic DNA. The HIST1H3A gene encodes one of the histone H3 variants, and its lysine 4 residue undergoes multiple methylation states, including mono, di, and tri methylation, each with distinct biological roles. Histone H3 (di methyl K4) antibody enables precise detection of this modification, providing insights into chromatin dynamics and transcriptional regulation across development and disease.Di methylation of histone H3 at lysine 4 is often enriched at promoter regions of actively transcribed genes. While tri methylation of lysine 4 strongly marks active promoters, di methylation provides a broader distribution pattern, sometimes marking both active and poised regulatory regions. Studies using Histone H3 (di methyl K4) antibody have revealed its involvement in the recruitment of chromatin remodeling complexes and transcription factors, linking it to fine tuning of gene activation. This modification is placed by SET domain containing methyltransferases, including the MLL/SET1 family, and removed by specific demethylases. The dynamic balance of methylation and demethylation at lysine 4 ensures flexibility in gene expression responses.Dysregulation of histone H3 lysine 4 methylation is implicated in cancer, developmental syndromes, and neurological disorders. Aberrant patterns of di methylation at promoters can alter transcriptional programs, driving oncogenesis or impairing differentiation. For example, mutations in methyltransferases such as MLL2 disrupt normal H3K4 methylation patterns, resulting in abnormal gene expression signatures in leukemia and solid tumors. Research with Histone H3 (di methyl K4) antibody has been critical for characterizing these altered epigenetic landscapes and understanding their impact on disease progression.Histone H3 (di methyl K4) antibody is widely used in chromatin immunoprecipitation, western blotting, and immunofluorescence. Chromatin immunoprecipitation coupled with sequencing (ChIP seq) allows genome wide mapping of histone modification patterns, revealing regulatory regions associated with transcriptional control. Western blotting confirms global levels of modification, while immunofluorescence highlights distribution within nuclear chromatin. These applications enable researchers to integrate molecular, genomic, and cellular approaches to study histone modification biology.Beyond oncology, Histone H3 (di methyl K4) antibody has applications in developmental biology and stem cell research. This modification marks regulatory elements required for lineage commitment and differentiation, making it an important indicator of epigenetic states during cell fate transitions. It is also studied in neurobiology, where changes in histone methylation accompany activity dependent gene expression and synaptic plasticity. By providing high quality reagents such as Histone H3 (di methyl K4) antibody, NSJ Bioreagents supports efforts to decode the epigenetic language of chromatin and its role in physiology and pathology.