Anti-Histone H2AS1Ph Monoclonal Antibody (RM216)

Histone H2A is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei. Histones are globular proteins with unstructured N-terminal tails and are subject to a variety of post-translational modifications, such as methylation, acetylation, phosphorylation, and citrullination, that can influence chromatin structure and regulate gene transcription. Phosphorylation of histone H2A at serine 1 (H2AS1Ph) is mediated by p90 ribosomal S6 kinase 5 (RSK5) and is associated with transcriptional repression. H2AS1Ph is enriched during mitosis in C. elegans, Drosophila, and HeLa cells, as well as during meiotic recombination in mouse spermatozoa. Cayman's Histone H2AS1Ph Monoclonal Antibody (RM216) can be used for ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications. This antibody is cross-reactive with histone H4 phosphorylated at serine 1 (H4S1Ph).Synonyms: H2ApS1, H2ApSer1, H2A (Phospho-Ser1), Phospho-Histone H2A Serine 1, Phosphorylated Histone H2A Serine 1. Immunogen: Peptide corresponding to H2AS1Ph. Clone Designation: RM216. Formulation: (Request formulation change), 100 µg of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ELISA, ICC, multiplex-based assays, WB. Cross Reactivity: (+) H2AS1Ph, (+) H4S1Ph, (-) Unmodified H2AS1, (-) Unmodified H4S1.