Anti-Histone H2A (C-Term) Monoclonal Antibody (Clone RM225)

Histone H2A is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei. It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to a variety of post-translational modifications (PTMs), including ubiquitination, acetylation, methylation, and phosphorylation, which function as epigenetic regulators of transcription. Histone H2A contains a C-terminal tail that, in contrast to other core histones, also extends outside of the nucleosome core and is highly variable, yielding a number of H2A variants that are subject to PTMs and differentially regulate nucleosome stability and chromatin structure. Serum histone H2A autoantibodies have been found in patients with drug-induced or spontaneous systemic lupus erythematosus (SLE). Cayman's Histone H2A (C-Term) Monoclonal Antibody (Clone RM225) can be used for ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications. This antibody recognizes the C-terminal region of histone H2A independent of PTMs.Immunogen: Peptide from the C-terminal region of human H2A. Clone Designation: RM225. Formulation: (Request formulation change), 100 µg of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ELISA, ICC, multiplex-based assays, WB. Cross Reactivity: (+) H2A independent of PTMs, (-) H2B, other histone proteins.