Anti-HES7 / Hairy and enhancer of split-related protein 7

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. HES7 antibody detects Hairy and enhancer of split-related protein 7, encoded by the HES7 gene on chromosome 17p13.1. HES7 is a basic helix-loop-helix (bHLH) transcriptional repressor and part of the HES family of Notch effectors. It plays a crucial role in the segmentation clock during somitogenesis, regulating cyclic gene expression that establishes somite boundaries in vertebrate embryos. HES7 functions by binding DNA at N-box and E-box elements, repressing transcription of target genes, including its own promoter, generating negative feedback loops essential for oscillatory expression. These oscillations underlie proper segmentation of the presomitic mesoderm into somites, which give rise to vertebrae and ribs.Disruption of HES7 expression results in severe developmental defects. Mutations in HES7 are associated with spondylocostal dysostosis, a congenital condition characterized by vertebral segmentation abnormalities and rib malformations. Mouse models confirm that loss of HES7 leads to disrupted somite boundaries and vertebral defects. At the cellular level, HES7 interacts with other transcription factors and repressors to coordinate Notch, Wnt, and FGF pathway signals in the segmentation clock network.Beyond development, aberrant HES7 expression has been detected in certain cancers, where altered Notch signaling contributes to tumorigenesis. As a transcriptional repressor, HES7 influences differentiation decisions, and dysregulation can promote uncontrolled proliferation. HES7 antibody is therefore valuable for developmental biology studies and for investigating the intersection of Notch signaling with disease pathways.Experimentally, HES7 antibody is used in immunoblotting, immunohistochemistry, and immunofluorescence to track oscillatory expression patterns in embryonic tissues. It also aids in studying Notch-mediated gene regulatory networks, cyclic transcriptional feedback, and congenital mutation effects. In cancer biology, it helps assess HES7 misexpression and its link to proliferative signaling pathways. NSJ Bioreagents provides HES7 antibody to ensure high-quality detection of this oscillatory transcription factor across research applications.