Anti-Hepsin (TMPRSS1)

Hepsin (Transmembrane protease serine 1) is a 51kD member of the peptidase S1 family of enzymes. It is expressed by both hepatocytes and numerous tumor cells, and activates HGF plus pro-uPA. Human hepsin is a type II transmembrane protein that contains a 23aa N-terminal cytoplasmic region and a 373aa extracellular domain (ECD) (aa45-417). The ECD possesses a SRCR segment (aa54-151) and a peptidase S1 domain (aa163-405). over aa46-417, human Hepsin shares 90% and 96% aa identity with mouse and canine hepsin, respectively. Hepsin may play an essential role in cell growth and maintenance of cell morphology. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 25ug/ml, ELISA: 0.5-1ug/ml, Optimal dilutions to be determined by the researcher. Hybridoma: Mouse myeloma cells with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human Hepsin (aa163-417). Storage and Stability: Lyophilized powder may be stored at -20°C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.