Anti-Heat Shock Protein 101 (Hsp101/ClpB- C-terminal)

Hsps/chaperones are known to be expressed in plants not only when they experience high temperatures stress, but also in response to a wide range of other environmental stresses such as water stress, salt stress, cold stress and oxidative stress (2, 3). Hsp100 is one such major class that acts as a chaperone. Hsp101/ClpB is a member of this Hsp100 family. These proteins help dissociate protein aggregates formed during heat stress to allow them to be , refolded by other chaperones. In spite of expression during heat stress, members of Hsp100 protein family are also expressed during seed development. Hsp101 protein is both nuclear and cytoplasmic-localized (1, 4). , , Applications:, Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot:1:2000, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.