Anti-HAND1 / Heart and neural crest derivatives-expressed protein 1

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. HAND1 antibody detects Heart and neural crest derivatives-expressed protein 1, a basic helix-loop-helix (bHLH) transcription factor that plays a central role in cardiac morphogenesis, placental development, and cell differentiation. The UniProt recommended name is Heart and neural crest derivatives-expressed protein 1 (HAND1). This transcription factor regulates gene expression programs that control cardiac chamber formation, trophoblast invasion, and embryonic patterning.Functionally, HAND1 antibody identifies a 215-amino-acid nuclear protein that binds E-box DNA motifs (CANNTG) as a heterodimer with other bHLH transcription factors such as E12 and HAND2. HAND1 activates or represses transcription depending on partner interactions and chromatin context. During embryogenesis, HAND1 is expressed in the developing heart, extraembryonic tissues, and craniofacial mesenchyme, where it directs differentiation of myocardial, trophoblast, and smooth muscle lineages.The HAND1 gene is located on chromosome 5q33.2 and is transcriptionally regulated by cardiac transcription factors including NKX2-5, GATA4, and MEF2C. HAND1 functions in a dosage-sensitive manner, as either loss or overexpression disrupts normal cardiac looping and ventricular formation. It acts downstream of BMP and WNT signaling pathways that orchestrate early mesoderm and cardiac progenitor specification.In the heart, HAND1 contributes to left ventricular development and patterning of the outflow tract. It regulates expression of structural and contractile genes required for cardiomyocyte differentiation. In the placenta, HAND1 promotes trophoblast giant cell differentiation and invasion, critical for maternal-fetal exchange. Beyond development, HAND1 has been implicated in cancer biology, where aberrant expression can influence cell proliferation, migration, and angiogenesis through transcriptional reprogramming of developmental pathways.HAND1 antibody is widely used in developmental biology, cardiology, and transcriptional regulation research. It is suitable for immunohistochemistry, immunofluorescence, and western blotting to detect HAND1 expression in embryonic and differentiated tissues. This antibody supports studies of cardiac gene regulation, lineage specification, and embryonic morphogenesis. It also aids in placental biology research and models of congenital heart defects.Structurally, HAND1 contains a basic DNA-binding region and a helix-loop-helix dimerization motif that enable sequence-specific DNA binding and partner selection. Post-translational modifications such as phosphorylation influence HAND1 dimerization and transcriptional output. NSJ Bioreagents provides HAND1 antibody reagents validated for use in cardiac development, trophoblast differentiation, and transcriptional network research.