Anti-GRIA4 (Glutamate Receptor 4, GluR-4, GluR4, AMPA-selective Glutamate Receptor 4, GluR-D, Glutam

Human glutamate receptor 4 (GRIA4) is a new member of the family of ionotropic glutamate receptors which are the predominant excitatory neurotransmitter receptors in the mammalian brain. Binding studies showed that human GRIA4 transfected into simian kidney cells (COS-1) exhibits high specific binding for[3H](RS)-alpha-amino- 3-hydroxy-5-methylisoxazole-4-propionic acid ([3H]AMPA) but not [3H]kainate. Ion substitution experiments indicate that hGluR4 receptor-linked ion channels in their homomeric state are permeable to both CA2+ and Na+ ions. Immunoprecipitation studies suggest that GRIA1 exists in situ in the form of a pentamer. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:128,000, Western Blot: 0.3-1ug/ml, observed in human brain (cerebellum) lysates on ~90kD bands, Immunohistochemistry (Paraffin): 5-10ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.