Anti-Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), clone 3H2171

GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages. It promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity. Applications: Suitable for use in ELISA, Immunofluorescence and Flow Cytometry. Other applications not tested. Recommended Dilution: Immunofluorescence: Useful for intracytoplasmic staining., Flow Cytometry: Identifies GM-CSF-positive cells within mixed cell populations., Optimal dilutions to be determined by the researcher. Control: I1904--82L4: Rat IgG2a , Storage and Stability:, May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.