Anti-GPR124

caution:It is uncertain whether Met-1 or Met-8 is the initiator.,domain:Binds to DLG1 through its PDZ-binding motif.,function:Orphan receptor.,similarity:Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.,similarity:Contains 1 GPS domain.,similarity:Contains 1 Ig-like (immunoglobulin-like) domain.,similarity:Contains 5 LRR (leucine-rich) repeats.,subunit:Interacts with DLG1.,tissue specificity:Expressed in endothelial cells (at protein level). Abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon., Protein function: Endothelial receptor which functions together with RECK to enable brain endothelial cells to selectively respond to Wnt7 signals (WNT7A or WNT7B) (PubMed:28289266, PubMed:30026314). Plays a key role in Wnt7-specific responses, such as endothelial cell sprouting and migration in the forebrain and neural tube, and establishment of the blood-brain barrier. Acts as a Wnt7-specific coactivator of canonical Wnt signaling: required to deliver RECK-bound Wnt7 to frizzled by assembling a higher-order RECK-ADGRA2-Fzd-LRP5-LRP6 complex (PubMed:30026314). ADGRA2-tethering function does not rely on its G-protein coupled receptor (GPCR) structure but instead on its combined capacity to interact with RECK extracellularly and recruit the Dishevelled scaffolding protein intracellularly (PubMed:30026314). Binds to the glycosaminoglycans heparin, heparin sulfate, chondroitin sulfate and dermatan sulfate (PubMed:16982628). [The UniProt Consortium] Recommended dilutions: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.. Cellular localization: Cell membrane , Multi-pass membrane protein . Cell projection, filopodium . Enriched at lateral cell borders and also at sites of cell-ECM (extracellular matrix) contact. .