Anti-GMII

Make to order. Production time: 70-80 business days. Deliverables: 1. 200 µg antigen (used as positive control), 2. 1 ml pre-immune serum (used as negative control) , 3. Rabbit polyclonal antibody, antigen affinity purified. Quality Guarantee: 1. Antibody purity > 90% confirmed by SDS-PAGE, 2. Antibody titer > 1: 64,000 confirmed by ELISA, 3. WB positive with the immunogen protein (not applicable for synthetic peptide). Applications: ELISA, WB (positive for the recombinant immunogen protein/peptide). Buffer: Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, pH 7.4. Protein function: Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N- glycans, the final hydrolytic step in the N-glycan maturation pathway. Converts GlcNAcMan(5)GlcNAc(2) (Man5Gn) into GlcNAcMan(3)GlcNAc(2) (MGn) by sequential removal of two alpha1,6- and alpha1,3-linked mannose residues from the alpha1,6-mannose branch of the substrate. To a lesser extent, also able to cleave beta1,2-xylosylated Man5Gn- glycopeptide (Man5GnX-GP) and pyridylaminated substrates Man5Gn-PA and Man5GnX-PA, but not active toward Man5-glycopeptide (PubMed:16460512, PubMed:21478158). Required for resistance to salt stress (PubMed:18408158). [The UniProt Consortium]