Anti-Glypican 3 / GPC3, clone GPC3/12698

1 mg/ml in 1X PBS, BSA free, sodium azide free. GPC3 antibody targets Glypican-3, a cell surface heparan sulfate proteoglycan encoded by the GPC3 gene and anchored to the plasma membrane through a glycosylphosphatidylinositol linkage. Glypican-3 is a member of the glypican family, which consists of extracellular matrix-associated proteoglycans that regulate growth factor signaling at the cell surface. Under normal physiological conditions, Glypican-3 expression is developmentally regulated and prominent during embryogenesis, where it contributes to tissue patterning, cell proliferation, and organ growth.Glypican-3 plays a key modulatory role in several major signaling pathways, including Wnt, Hedgehog, fibroblast growth factor, and insulin-like growth factor signaling. By binding growth factors and morphogens through its heparan sulfate chains, Glypican-3 influences ligand availability and receptor activation at the cell membrane. This regulatory function positions Glypican-3 as an important determinant of cell fate decisions and proliferative capacity during development. A Glypican-3 antibody is therefore useful for studying cell surface signaling regulation and developmental biology mechanisms.In adult tissues, Glypican-3 expression is typically low or absent, but it becomes strongly re-expressed in specific disease contexts. Most notably, Glypican-3 is highly expressed in hepatocellular carcinoma, where it is considered a hallmark oncofetal protein. Elevated Glypican-3 levels are also reported in certain pediatric tumors and germ cell-derived malignancies. Because of this restricted expression pattern, GPC3 antibody reagents are widely used in cancer research to study tumor cell identity, differentiation state, and aberrant growth signaling.Glypican-3 is synthesized as a precursor protein that undergoes post-translational processing, including proteolytic cleavage and heparan sulfate chain modification. These processing steps generate multiple Glypican-3 species that may differ in apparent molecular size and biological activity. Use of a Glypican-3 antibody enables investigation of these processing events and their relationship to tumor-associated signaling changes. In tissue sections, Glypican-3 is typically detected at the cell surface and within the cytoplasm, reflecting biosynthetic trafficking and membrane localization dynamics.From a research and diagnostic perspective, Glypican-3 has become one of the most extensively studied biomarkers in liver cancer biology. GPC3 antibody staining is commonly used to distinguish hepatocellular carcinoma from benign hepatic lesions and metastatic tumors in experimental and translational studies. In addition, Glypican-3 has attracted interest as a therapeutic target, further increasing the importance of well-characterized antibodies for mechanistic and preclinical research.Clone GPC3/12698 is designed to recognize Glypican-3 and supports detection of this oncofetal proteoglycan in research applications. NSJ Bioreagents offers this GPC3 antibody to support investigations into liver cancer biology, developmental signaling pathways, and cell surface proteoglycan function.