Anti-GLYAT / Glycine-N-acyltransferase

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. GLYAT antibody detects Glycine-N-acyltransferase, encoded by the GLYAT gene on chromosome 11q12.1. GLYAT antibody is widely used to study this phase II conjugating enzyme involved in detoxification and metabolism of acyl-CoA thioesters. GLYAT catalyzes conjugation of glycine to acyl-CoA substrates, producing acylglycines that are more water-soluble and easily excreted. Expression is highest in liver and kidney, the major detoxification organs, but it is also present in brain and other tissues involved in metabolic processing.Structurally, GLYAT is a mitochondrial matrix enzyme belonging to the acyltransferase family. It contains conserved motifs for CoA binding and catalytic activity. Structural studies suggest GLYAT functions as a homodimer, with active sites capable of accommodating medium- to long-chain acyl-CoA substrates. This structural adaptability allows it to process a wide range of xenobiotic and endogenous compounds.Functionally, GLYAT contributes to detoxification of acyl-CoA intermediates generated during fatty acid, amino acid, and xenobiotic metabolism. By conjugating glycine to these intermediates, GLYAT prevents their accumulation and toxicity. Acylglycines produced by GLYAT can serve as diagnostic markers of inborn errors of metabolism, including organic acidemias. Knockdown studies demonstrate impaired detoxification capacity and increased cellular stress. Researchers use GLYAT antibody to study metabolic regulation, detoxification, and biomarker pathways.Clinically, GLYAT deficiency or reduced activity is associated with metabolic disorders characterized by elevated toxic acyl-CoA levels. Acylglycine profiling is used in newborn screening for metabolic diseases such as isovaleric acidemia. GLYAT expression has also been linked to cancer, where metabolic reprogramming alters detoxification pathways. Additionally, GLYAT is implicated in drug metabolism and may influence individual responses to xenobiotics. NSJ Bioreagents offers GLYAT antibody as a research tool in toxicology, metabolism, and disease studies.Experimentally, GLYAT antibody is applied in western blotting to detect the ~34 kDa protein, in immunofluorescence microscopy to confirm mitochondrial localization, and in immunohistochemistry to analyze tissue expression. Enzymatic assays combined with GLYAT antibody detection help characterize acyltransferase activity under different conditions.