Anti-GLI3 (GLI3 form of 190kD)

GLI3(Gli-kruppel family member 3), also called ONCOGENE GLI3, encodes a member of the zinc , finger gene family related to Kruppel, a gene that is known to regulate development in Drosophila., The GLI3 gene is expressed as an 8.5-kb mRNA in tissues such as testis, myometrium, placenta, and , lung, and the protein product (relative molecular mass, 190,000) shows sequence-specific DNA binding. , The GLI3 gene is mapped on 7p14.1. GLI3 is homologous to the Drosophila cubitus interruptus (ci) , gene product (Ci), which regulates the Patched (pct), gooseberry (gsb), and decapentaplegic (dpp) , genes. PKA-dependent processing of vertebrate GLI3 in developing limb generates a potent repressor , in a manner antagonized by apparent long-range signaling from posteriorly localized Sonic hedgehog , protein. The high relative abundance and potency of GLI3 repressor suggested specialization , of GLI3 and its products for negative hedgehog pathway regulation.Coimmunoprecipitation and , immunoblot studies showed that GLI3 protein is polyubiquitinated and that its processing depends on , proteasome activity. The findings suggested that BTRC is required for GLI3 processing. UniProt Number: P10071, Gene ID: GLI3, Applications: Suitable for use in Western Blot. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Handling: Store at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term., Avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.