Anti-GKN1 (Gastrokine-1, 18 kDa Antrum Mucosa Protein, AMP-18, Protein CA11, AMP18, CA11, Gastrokine

Gastrokine 1 is an 18kD member of the CA11 protein family. It has limited expression, being restricted to mucous secreting pyloric atrial epithelium. Gastrokine 1 appears to promote epithelial proliferation and migration, and induce the formation of tight junctions between epithelial cells. Mature human Gastrokine 1 is 165aa in length. Based on the SwissProt sequence, it possesses one BRICHOS domain aa54-150 that contains a mitogenic sequence aa97-117. There is one potential alternative start site 14 aa upstream of the standard site that does not appear to be utilized. Over aa21-185 (aa35-199 of SwissProt Q9NS71), human Gastrokine 1 shares 65aa identity with mouse Gastrokine 1. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.