Anti-GK2 / Glycerol kinase 2

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. GK2 antibody detects Glycerol kinase 2, encoded by the GK2 gene on chromosome 4q13.3. GK2 antibody is commonly applied in studies of glycerol metabolism, mitochondrial energy regulation, and sperm function. GK2 belongs to the glycerol kinase family, enzymes that phosphorylate glycerol to glycerol-3-phosphate, a critical intermediate in lipid metabolism and glycolysis. Unlike the ubiquitously expressed GK1, GK2 is primarily expressed in testis and sperm, where it plays a role in fertility and energy production. It localizes to mitochondria, highlighting its role in coupling glycerol metabolism with energy pathways.Structurally, GK2 is a ~61 kDa enzyme composed of conserved ATP-binding and substrate-binding domains typical of kinases in this family. It lacks some regulatory motifs present in GK1, leading to distinct enzymatic properties and tissue specificity. The mitochondrial localization sequence targets GK2 to the inner membrane, enabling direct integration into oxidative phosphorylation networks.Functionally, GK2 catalyzes the phosphorylation of glycerol to glycerol-3-phosphate, fueling glycolysis and triglyceride synthesis. In sperm, this activity supports energy metabolism necessary for motility and fertilization. GK2 also contributes to mitochondrial glycerol-3-phosphate shuttle activity, linking cytosolic NADH reoxidation with the respiratory chain. Knockdown or disruption of GK2 leads to impaired sperm motility and male infertility, emphasizing its critical reproductive role. Researchers use GK2 antibody to study sperm metabolism, mitochondrial function, and lipid biochemistry.Clinically, GK2 mutations or dysfunction have been associated with infertility and metabolic syndromes involving lipid dysregulation. Because GK2 expression is highly specific to testis, it has been investigated as a biomarker of male fertility potential. GK2 is also implicated in mitochondrial biology disorders, where energy coupling is impaired. Its restricted expression compared to GK1 makes it an attractive target for reproductive health research. NSJ Bioreagents provides GK2 antibody as a validated tool for mitochondrial and fertility research.Experimentally, GK2 antibody is applied in western blotting to detect the ~61 kDa protein, in immunofluorescence microscopy to localize it in mitochondria, and in immunohistochemistry to examine expression in testis tissue. Enzyme assays combined with GK2 antibody detection enable correlation of enzymatic activity with localization and expression.