Anti-GJA4 / Gap junction alpha 4

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. GJA4 antibody detects Gap junction alpha-4 protein, also known as Connexin 37, a transmembrane protein forming intercellular channels that mediate direct communication between adjacent cells. GJA4 enables diffusion of ions, metabolites, and signaling molecules, maintaining vascular tone and tissue homeostasis. The GJA4 antibody is widely used in cardiovascular, reproductive, and cellular communication studies to explore gap junction biology and endothelial signaling.GJA4 is encoded by the GJA4 gene on human chromosome 1p35.1. The protein is approximately 333 amino acids long and contains four transmembrane helices, two extracellular loops, and cytoplasmic N- and C-terminal domains. GJA4 assembles into hexameric connexons that dock with connexons of neighboring cells, forming gap junction channels that permit electrical and chemical coupling.The GJA4 antibody detects a 37 kilodalton band by western blot and shows punctate membrane staining consistent with gap junction plaques under immunofluorescence. GJA4 is abundantly expressed in vascular endothelial and smooth muscle cells, where it mediates synchronized contraction and vascular tone regulation. In ovarian follicles and the placenta, GJA4 facilitates cell communication critical for follicular development and embryonic exchange.Mutations in GJA4 disrupt gap junction communication, contributing to vascular dysfunction and reproductive disorders. Altered GJA4 expression has been observed in atherosclerosis, hypertension, and tumor angiogenesis. Its expression pattern varies with hormonal and mechanical stimuli, reflecting its responsiveness to physiological cues controlling vascular integrity.GJA4 channels are regulated by phosphorylation, pH, and redox state, allowing dynamic modulation of intercellular connectivity. Because gap junctions coordinate tissue-level responses, GJA4 represents a vital component of multicellular signaling networks. NSJ Bioreagents provides a validated GJA4 antibody optimized for its applications, supporting detailed studies of cell coupling, signal propagation, and endothelial homeostasis.