Anti-GATA3 (GATA3, GATA binding protein 3, HDR, MGC2346, MGC5199, MGC5445, GATA-binding protein 3, e

Alternate Name: GATA3, GATA binding protein 3, HDR, MGC2346, MGC5199, MGC5445, GATA-binding protein 3, enhancer-binding protein GATA3, Domain Information: zinc finger binding to DNA consensus sequence [AT]GATA[AG] , Gene Ontology Terms: DNA binding, transcription activating factor, transcription factor activity, embryogenesis and morphogenesis, defense response, transcription from Pol II promoter, Application(s): Suitable for use in ELISA, Western Blot. Other applications not tested. Western blot: Approx 45-50kD band seen in MOLT-4 lysate. Recommended for use at 2-4ug/ml. Staining (3ug/ml) of MOLT-4 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence. , Immunohistochemistry: In paraffin embedded Human Tonsil, stains nuclei of some cells in the interfollicular area and in germinal centres. Recommended concentration, 0.1-0.3µg/ml., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See G2021-49-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.