Anti-Galectin-3 (GAL3, Carbohydrate Binding Protein 35, CBP35, Galactose Specific Lectin 3), clone 4

Galectins are a new family of animal lectins which appear to exhibit a variety of biological functions and may in fact be a broad-spectrum biological response modifier. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homooligomerized. Among its numerous proposed functions, Galectin 3 may be a component of a cell-growth regulating system given its elevated expression and nuclear localization in proliferating cells. It has also been found to be a major non-integrin laminin-binding protein suggesting a role in cell adhesion. One of its more interesting proposed functions is as an inhibitor of apoptosis. Galectin 3 shares an NWGR motif with Bcl-2 which has been shown to be an imjportant region in Bcl-2 for suppression of apoptosis. Galectin 3 has been shown to interact with Bcl-2 which may account for the ability of Galectin 3 to suppress programmed cell death in anti-Fas treated Jurkat cells transfected with Galectin 3. In contrast to Galectin 3, Galectin 1 actually induces apoptosis when added to cultures of activated human T cells. Applications: Suitable for use in Immunohistochemistry, Flow Cytometry, Western Blot,Immunofluorescence/Immunocytochemistry and ELISA. Other applications not tested. Recommended Dilutions: ELISA: 1:100-1:2000, Flow Cytometry: 1:100-1:2000, Immunohistochemistry: 1:10-1:500, Immunofluorescence (IC):1:10-1:500, Immunohistochemistry: (Paraffin): 6ug/ml , Immunohistochemistry: (Frozen): 6ug/ml, Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Positive Control: Rat Olfactory bulb, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.