Anti-G6B (Protein G6b, C6orf25)

G6B is a 241aa novel regulatory nuclear chloride ion channel protein belonging to the immunoglobulin (Ig) super family. It is a glycosylated receptor, present both as soluble and transmembrane isoforms where the soluble proteins show expression in endoplasmic reticulum and Golgi before being secreted. It contains a leader sequence and an extracellular Ig domain which binds to heparin and helps to modulate the interaction of G6B with an unknown protein ligand. It also has an intracellular, proline-rich potential SH3-binding domain, and two putative phosphorylated tyrosine motifs with an SH2-binding domain that is recruited to the receptor and dephosphorylates membrane-bound phosphoproteins and thus modulates the signaling cascade. It is commonly expressed in certain hematopoietic cell lines including K562, Molt4, and Jurkat. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml, Optimal dilutions to be determined by the researcher. Positive Control: K562 cell lysate, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.