Anti-Frizzled-1 (FZD1), clone 5A1

Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway and are involved in a variety of developmental processes. Frizzled proteins each contain a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain, a variable-length linker region, a sevenpass transmembrane domain and a variable-length C-terminal tail. Applications: Suitable for use in Western Blot, Flow Cytometry and Direct ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml. The detection limit is for recombinant mouse Frizzled-1 is ~5ng/lane and ~25ng/lane under non-reducing and reducing conditions, respectively., Flow Cytometry: 0.1mg/ml, Add 10ul to 1-2.5x10e5 cells in a total reaction volume not exceeding 200ul. , Direct ELISA: 0.5-1.0ug/ml. The detection limit for recombinant mouse Frizzled-1 is ~10ng/well., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.