Anti-FOXP4 (Forkhead Box Protein P4, Fork Head-related Protein-like A, FKHLA)

FoxP4 is a 70-80kD member of the winged helix transcription factor gene family, FoxP subfamily of proteins. It is a transcriptional repressor that is strongly expressed in embryonic gut and lung epithelium, and in neurons both pre-and postnatally. Postnatally, it is found in cerebellar Purkinje cells. During development, it is found in hippocampal hilus, proliferating cells of the ventricular zone, and neurons of the basolateral nucleus of the amygdala. Human FoxP4 is 680aa in length. It contains a large poly-Gln region aa65-219, a C2H2 Zn-finger domain aa307-332, a Leu-zipper dimerization motift aa349-370 and a forkhead DNA binding domain aa467-559. Phosphorylation occurs at Ser360. FoxP4 forms homodimers, and heterodimers with FoxP1 and FoxP2, it does not bind CTBP1. One potential splice form shows a deletion of aa384-395. Over aa549-680, human and mouse FoxP4 share 89% aa identity. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.