Anti-FOXM1 / Forkhead box protein M1

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. FOXM1 antibody detects Forkhead box protein M1, encoded by the FOXM1 gene on chromosome 12p13.33. FOXM1 antibody is widely used in studies of cell cycle regulation, transcription, and cancer biology. FOXM1 is a member of the forkhead box (FOX) family of transcription factors, characterized by a conserved winged-helix DNA-binding domain. It plays critical roles in G1/S and G2/M transitions of the cell cycle, controlling genes required for DNA replication, mitosis, and cytokinesis. Expression is high in proliferating cells and embryonic tissues, but normally downregulated in differentiated, non-dividing cells.Structurally, FOXM1 contains an N-terminal repressor domain, a central forkhead DNA-binding domain, and a C-terminal transactivation domain. Its activity is regulated by phosphorylation, with cyclin-CDK complexes and MAPKs activating FOXM1 during the cell cycle. Alternative splicing produces isoforms with distinct transcriptional activities, including FOXM1a (inactive), FOXM1b, and FOXM1c (active in proliferation).Functionally, FOXM1 drives expression of cell cycle regulators such as cyclin B1, cyclin D1, PLK1, and Aurora B kinase. It ensures proper chromosome segregation, spindle assembly, and DNA repair. Loss of FOXM1 impairs proliferation and causes mitotic defects, while overexpression accelerates tumorigenesis. Researchers use FOXM1 antibody to study transcriptional regulation, cell cycle control, and tumor progression.Clinically, FOXM1 is strongly associated with cancer. Overexpression occurs in breast, liver, prostate, lung, and brain tumors, correlating with aggressive phenotypes and poor prognosis. FOXM1 promotes tumor growth by enhancing proliferation, metastasis, angiogenesis, and therapy resistance. Targeting FOXM1 is an emerging therapeutic strategy, with inhibitors under development. Beyond oncology, FOXM1 also participates in tissue repair, stem cell biology, and aging processes. NSJ Bioreagents provides FOXM1 antibody for use in oncology, stem cell, and regenerative medicine research.Experimentally, FOXM1 antibody is applied in western blotting to detect the ~80?90 kDa protein, in immunohistochemistry to assess tumor expression, and in immunofluorescence microscopy to study nuclear localization. ChIP assays using FOXM1 antibody help identify direct gene targets of this transcription factor.